The experiment of Avery and his collaborators Colin MacLeod and Maclyn McCarty was conducted in 1943 and is one of the key experiments in advancing knowledge of genetics and molecular biology. This experiment seeks to understand the "transformation" that Griffith had previously discovered. In this way he used the following methodology:
First of all, the dead S-type bacteria were collected in a container which was closed.
Then you performed a cell extraction of everything except DNA, the proteins, and RNA. Where you added RNase (to kill RNA) to the first beaker, protease (to remove proteins) to the second beaker, and finally DNase (to remove DNA) to the third beaker.
After this, as it appears in the image, each glass is added to a colony of R Vives, in this way we could see which of the three assets acted as a modifier of genetic material.
With this experiment we can see that DNA is the transforming principle, which means that at the same time it has DNA inside it and therefore it is shown that it is not the proteins that store it. Concluding that genes, or molecules that dictate how organisms develop, are made of deoxyribonucleic acid, or DNA.
